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Ujwal

Professor

Title: Diagnosis of MDR-TB directly on Sputum positive specimens in PLHIVs by rapid Molecular method.

Biography

Biography: Ujwal

Abstract

Background: The control of tuberculosis infection in PLHIVs has now become more complicated due to emerging of multidrug resistant TB (MDR-TB). It takes longer time; at least 6-8weeks for diagnosis of MDR-TB by culture and conventional DST. The aim of this study was to early diagnosis of MDR-TB by rapid molecular method directly from positive sputum specimens to decrease the mortality of PLHIVs.

Method: A total of 25 smear positive sputum specimens of PLHIVs were used for the detection of RIF and INH resistant mutation by genotypic assay (Genotype MTBDR plus kit, Hain Life science GmbH, Nehren, Germany) and compared with phenotypic DST from the same specimens.

Result: Out of the 25 specimens, an interpretable result of MDR-TB (RIFr+INHr), were obtained from 7/23(30.4%), by Genotypic method and 8/23(34.7%) from phenotypic method. The majority of common mutation regions were seen in MUT3 64.3% (Ser531lue) in rpoB gene, MUT1 24.5% (Ser315Ile) in katG gene and MUT1 5.6% (Cys-15Thr) in inhA gene.

Conclusion:This method is suitable for rapid diagnosis of MDR-TB, directly on smear positive sputum specimens in PLHIVs because it is equally sensitive and specific and takes less time as compared with conventional DST.